|About miRNA Designer
is a web tool developed by Dr. Guiliang Tang at Michigan Technological University in collaboration with
Dr. Chingwen Li. It was targeted for developing
an NSF Plant Genome Proposal as well as an NIH proposal for
general human health. As a proof of concept, we constructed an miRDuBase
(miRNA duplex database) that contains more than 40 biological species.
A large data set from these species has been stored in the miRDuBase
for computer modeling which leaded to a rational miRNA designing that
does not simply mimic any specific miRNA structure. Pre-miRNA-like
structures, as an output of this program, will be subjected to extensive
in vitro process by using plant and Drosophila embryo extracts.
The rules of those structures that have the highest efficiency in production
of mature miRNAs will be used for optimizing the miRNA designer.
Pre-liminary experimental results have proven that this strategy is highly efficient
and the program output is reliable. The current version of miRNA Designer
will allow fellow scientists to test by choosing mismatches between the guider
and passenger strand of the miRNA duplex based on observed positional occurrence
among a variety of species. Comments and testing results can be adressed
Dr. Guiliang Tang at Michigan Technological