| miRNA End Energy Calculator |
| The miRNA End Energy calculator takes miRNA duplex to calculate free energy
for 5 base pairs at one end plus a dangling nucleotide, if there is any.
Following the tips below to enter the miRNA duplex: |
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- Select calculation for 5' end, 3' end or both.
- Enter each strand of the RNA sequence. Make sure two strands are in same length.
- Use "-" to fill the sequence if there is a dangling nucleotide in the opposite strand.
- Consecutive dangling nucleotides or mismatch pairs in the end are not allowed.
- Example: 5(-uaucacagccagcuuugaggag)3 and 3(cguagugucggucgaaacuccu-)5; -7.06 and -10.84 kcal/mol.
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